Replication of influenza A virus in swine umbilical cord epithelial stem-like cells
Identifieur interne : 000675 ( Main/Exploration ); précédent : 000674; suivant : 000676Replication of influenza A virus in swine umbilical cord epithelial stem-like cells
Auteurs : Mahesh Khatri [États-Unis] ; Kuldeep S. Chattha [Canada]Source :
- Virulence [ 2150-5594 ] ; 2014.
Abstract
In this study, we describe the isolation and characterization of epithelial stem-like cells from the swine umbilical cord and their susceptibility to influenza virus infection. Swine umbilical cord epithelial stem cells (SUCECs) expressed stem cell and pluripotency associated markers such as SSEA-1, SSEA-4, TRA 1–60 and TRA 1–81 and Oct4. Morphologically, cells displayed polygonal morphology and were found to express epithelial markers; pancytokeratin, cytokeratin-18 and occludin; mesenchymal cell markers CD44, CD90 and haematopoietic cell marker CD45 were not detected on these cells. The cells had extensive proliferation and self- renewal properties. The cells also possessed immunomodulatory activity and inhibited the proliferation of T cells. Also, higher levels of anti-inflammatory cytokine IL-10 were detected in SUCEC-T cell co-cultures. The cells were multipotent and differentiated into lung epithelial cells when cultured in epithelial differentiation media. We also examined if SUCECs are susceptible to infection with influenza virus. SUCECs expressed sialic acid receptors, used by influenza virus for binding to cells. The 2009 pandemic influenza virus and swine influenza virus replicated in these cells. SUCECs due to their differentiation and immunoregulatory properties will be useful as cellular therapy in a pig model for human diseases. Additionally, our data indicate that influenza virus can infect SUCECs and may transmit influenza virus from mother to fetus through umbilical cord and transplantation of influenza virus-infected stem cells may transmit infection to recipients. Therefore, we propose that umbilical cord cells, in addition to other agents, should also be tested for influenza virus before cryopreservation for future use as a cell therapy for disease conditions.
Url:
DOI: 10.4161/21505594.2014.983020
PubMed: 25517546
PubMed Central: 4603428
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Pmc, to step Corpus: 000026
- to stream Pmc, to step Curation: 000026
- to stream Pmc, to step Checkpoint: 000464
- to stream Ncbi, to step Merge: 000B14
- to stream Ncbi, to step Curation: 000B14
- to stream Ncbi, to step Checkpoint: 000B14
- to stream Main, to step Merge: 000676
- to stream Main, to step Curation: 000675
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Replication of influenza A virus in swine umbilical cord epithelial stem-like cells</title>
<author><name sortKey="Khatri, Mahesh" sort="Khatri, Mahesh" uniqKey="Khatri M" first="Mahesh" last="Khatri">Mahesh Khatri</name>
<affiliation wicri:level="1"><nlm:aff id="af0001"><institution>Food Animal Health Research Program; Ohio Agricultural Research and Development Center; The Ohio State University</institution>
; Wooster, OH<country>United States</country>
</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Chattha, Kuldeep S" sort="Chattha, Kuldeep S" uniqKey="Chattha K" first="Kuldeep S" last="Chattha">Kuldeep S. Chattha</name>
<affiliation wicri:level="1"><nlm:aff id="af0002"><institution>Canadian Food Inspection Agency</institution>
; Lethbridge, Alberta<country>Canada</country>
</nlm:aff>
<country xml:lang="fr">Canada</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PMC</idno>
<idno type="pmid">25517546</idno>
<idno type="pmc">4603428</idno>
<idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4603428</idno>
<idno type="RBID">PMC:4603428</idno>
<idno type="doi">10.4161/21505594.2014.983020</idno>
<date when="2014">2014</date>
<idno type="wicri:Area/Pmc/Corpus">000026</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000026</idno>
<idno type="wicri:Area/Pmc/Curation">000026</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000026</idno>
<idno type="wicri:Area/Pmc/Checkpoint">000464</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">000464</idno>
<idno type="wicri:Area/Ncbi/Merge">000B14</idno>
<idno type="wicri:Area/Ncbi/Curation">000B14</idno>
<idno type="wicri:Area/Ncbi/Checkpoint">000B14</idno>
<idno type="wicri:doubleKey">2150-5594:2014:Khatri M:replication:of:influenza</idno>
<idno type="wicri:Area/Main/Merge">000676</idno>
<idno type="wicri:Area/Main/Curation">000675</idno>
<idno type="wicri:Area/Main/Exploration">000675</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Replication of influenza A virus in swine umbilical cord epithelial stem-like cells</title>
<author><name sortKey="Khatri, Mahesh" sort="Khatri, Mahesh" uniqKey="Khatri M" first="Mahesh" last="Khatri">Mahesh Khatri</name>
<affiliation wicri:level="1"><nlm:aff id="af0001"><institution>Food Animal Health Research Program; Ohio Agricultural Research and Development Center; The Ohio State University</institution>
; Wooster, OH<country>United States</country>
</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Chattha, Kuldeep S" sort="Chattha, Kuldeep S" uniqKey="Chattha K" first="Kuldeep S" last="Chattha">Kuldeep S. Chattha</name>
<affiliation wicri:level="1"><nlm:aff id="af0002"><institution>Canadian Food Inspection Agency</institution>
; Lethbridge, Alberta<country>Canada</country>
</nlm:aff>
<country xml:lang="fr">Canada</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
</analytic>
<series><title level="j">Virulence</title>
<idno type="ISSN">2150-5594</idno>
<idno type="eISSN">2150-5608</idno>
<imprint><date when="2014">2014</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass></textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en"><p>In this study, we describe the isolation and characterization of epithelial stem-like cells from the swine umbilical cord and their susceptibility to influenza virus infection. Swine umbilical cord epithelial stem cells (SUCECs) expressed stem cell and pluripotency associated markers such as SSEA-1, SSEA-4, TRA 1–60 and TRA 1–81 and Oct4. Morphologically, cells displayed polygonal morphology and were found to express epithelial markers; pancytokeratin, cytokeratin-18 and occludin; mesenchymal cell markers CD44, CD90 and haematopoietic cell marker CD45 were not detected on these cells. The cells had extensive proliferation and self- renewal properties. The cells also possessed immunomodulatory activity and inhibited the proliferation of T cells. Also, higher levels of anti-inflammatory cytokine IL-10 were detected in SUCEC-T cell co-cultures. The cells were multipotent and differentiated into lung epithelial cells when cultured in epithelial differentiation media. We also examined if SUCECs are susceptible to infection with influenza virus. SUCECs expressed sialic acid receptors, used by influenza virus for binding to cells. The 2009 pandemic influenza virus and swine influenza virus replicated in these cells. SUCECs due to their differentiation and immunoregulatory properties will be useful as cellular therapy in a pig model for human diseases. Additionally, our data indicate that influenza virus can infect SUCECs and may transmit influenza virus from mother to fetus through umbilical cord and transplantation of influenza virus-infected stem cells may transmit infection to recipients. Therefore, we propose that umbilical cord cells, in addition to other agents, should also be tested for influenza virus before cryopreservation for future use as a cell therapy for disease conditions.</p>
</div>
</front>
</TEI>
<affiliations><list><country><li>Canada</li>
<li>États-Unis</li>
</country>
</list>
<tree><country name="États-Unis"><noRegion><name sortKey="Khatri, Mahesh" sort="Khatri, Mahesh" uniqKey="Khatri M" first="Mahesh" last="Khatri">Mahesh Khatri</name>
</noRegion>
</country>
<country name="Canada"><noRegion><name sortKey="Chattha, Kuldeep S" sort="Chattha, Kuldeep S" uniqKey="Chattha K" first="Kuldeep S" last="Chattha">Kuldeep S. Chattha</name>
</noRegion>
</country>
</tree>
</affiliations>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/H2N2V1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000675 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000675 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Sante |area= H2N2V1 |flux= Main |étape= Exploration |type= RBID |clé= PMC:4603428 |texte= Replication of influenza A virus in swine umbilical cord epithelial stem-like cells }}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:25517546" \ | HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \ | NlmPubMed2Wicri -a H2N2V1
This area was generated with Dilib version V0.6.33. |